POST-DOCTORAL POSITION at the Hennepin Healthcare Research Institute (HHRI)
and the Department of Nephrology at Hennepin Healthcare’s HCMC
About the Hennepin Healthcare Research Institute
The Hennepin Healthcare Research Institute (HHRI) in Minneapolis, Minnesota, invites applications for a Post-Doctoral Research Fellow to support the research being conducted in Hennepin Healthcare’s HCMC department of Nephrology.
At the HHRI, we have been actively investigating the causes of and potential treatments for diseases since 1952. We support and oversee the medical research conducted at Hennepin Healthcare System’s HCMC, an acute care research and teaching hospital located in downtown Minneapolis. In an academic environment that emphasizes patient care, research, and teaching, HHRI helps offer patients better care now and hope for improved healthcare in the future.
HHRI is the nonprofit 501(c)(3) institute responsible for providing administrative and fiscal oversight for research performed on the HCMC campus. The HHRI is one of the largest medical research institutes in Minnesota and consistently ranks in the top ten percent of all institutions receiving research funding from the National Institute of Health (NIH). HHRI administers over $23 million in federally funded activity with $40 million in total research funds annually.
HCMC is a 484-bed public teaching hospital located in downtown Minneapolis, MN. HCMC employs approximately 6,000 people and is Minnesota’s largest safety net hospital. HCMC conducts approximately 600,000 clinic visits annually and is the state’s premier Level 1 Trauma Center. The majority of HCMC patients are of minority racial and/or ethnic backgrounds
The Fellow will work in the Lab of Assistant Professor Casey Dorr, PhD and Professor Ajay Israni, MD, MS. This is a smaller lab group that is part of a large, dynamic multi-disciplinary genomics of transplantation research team of statisticians, pharmacologists, clinicians, nurses, molecular geneticists, human biologists and bioinformaticians. The Fellow will have the opportunity to become a genetic engineer specialist of this team and be responsible for moving an NIH funded, genomics and pharmacogenomics study forward with emphasis on drug metabolism in human subjects and bioengineered cell lines.
The Fellow will be given freedom to develop innovative strategies to carry out research design and data analysis and have ample opportunity for individualized mentoring and training.
This position will require extensive lab work making reagents and bioengineering human cell lines using CRISPR and other innovative technologies. Engineered cell lines will be used for drug metabolism and other pharmacogenomics studies. The candidate will be expected to keep up on the latest bioengineering technologies and other recent advances in molecular cellular biology.
The Fellow will also assist in development of new spatial transcriptomic and proteomic technologies along with molecular imaging of biopsy specimens. This will further include RNA-seq, whole transcriptome and next generation DNA/RNA sequencing studies. The majority of these technologies are currently being applied, in our lab, to make advancements in the field of solid organ transplantation and pharmacogenomics.
Worth noting, all lab personnel have been vaccinated against SARS-COV-2 and vaccination of the Fellow will be available upon employment. As the COVID pandemic subsides, this position will have regular travel between the HHRI lab and the University of Minnesota.
o Conduct and/or direct routine and complex research projects in a genetics epidemiological laboratory.
o Evaluate, suggest alternative methods, modify or develop new procedures and techniques to aid in the investigation of a total research problem or program
o Maintain records and large databases and analyzes research data
o Help maintain a molecular epidemiology and cell culture lab
o Although the Fellow will be mentored, the Fellow must be self-driven to conduct the projects and work independently.
o The fellow will develop and/or refine the following basic laboratory techniques:
o CRISPR engineering of human cells in cell culture
o Drug Metabolism assays
o Cell line characterization including DNA, RNA and Protein assays
o Variety of molecular biology techniques
o PCR, molecular cloning in E. Coli, DNA ligations, restriction enzyme mapping
o High throughput PCR techniques for screening human and plasmid DNA
o RT-PCR and RNA splicing assays
o The Fellow may assist in development of innovative studies that are being conducted in the lab. These studies may include drug metabolism studies, next-generation sequencing technologies such as single nucleus RNAseq, microbiome, whole genome/whole exome/targeted sequencing projects and single cell imaging.
o Plan new avenues of investigation and conduct investigations into research projects
o Communicate research findings to scientific community.
o Co-author and First author manuscripts for publications in peer-reviewed journals with the established scientific team
o The Fellow will be trained in writing skills as needed
o Draft proposals to procure research funding.
o The Fellow will have opportunity for continued learning and likely take short courses for targeted skill developments including writing, career development and advanced scientific techniques.
o The Fellow will be encouraged to engage in career development activities through conferences, seminars and through connection with the University of Minnesota.
o The Fellow will be encouraged to have (or develop) a long-term career vision.
The Fellow will work in a Nephrology Department and willingness to understand kidney diseases and transplantation is required.
Typical qualifications would be a PhD (or equivalent terminal degree) in human genetics, molecular cellular biology, microbiology, virology, genetic epidemiology or related field. Must be well-versed in molecular cellular biology including human (or other eukaryotic) cell culture and molecular cloning techniques. A background in drug metabolism enzymes (such as CYP3A4 and CYP3A5) and transporters is preferred. Understanding of how genetic variants influence phenotype will be essential. Experience in statistics and/or bioinformatics such as PCR and sequencing primer design, plasmid mapping, sequencing analysis is preferred.
Skill, Knowledge & Ability (SKA):
Requires interaction with a diverse population. Must demonstrate effective verbal and written communication skills and have proven organizational and documentation skills. Must possess a relevant science background and the ability to utilize an applied and investigatory foundation in natural science and evaluate given tasks. Ability to adhere to institutional safety protocols, established guidelines of laboratory policy and procedure, apply scientific methods, theories, and techniques. Displays high standards of attendance, punctuality, maintains confidentiality, and manages time effectively. Willingness to learn new laboratory techniques and innovative scientific strategies is essential.
This position will be compensated based on NIH guidelines for post-doctoral roles.
AA/EOE of Minorities, Women, Disabilities, Veterans
Questions about this position can be directed to Ajay Israni, M.D., M.S. (firstname.lastname@example.org)